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clpE [2018-02-12T20:42:45.000Z]
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clpE [2018-02-12T20:42:45.000Z]

AAA unfoldase, ATPase subunit of the ClpE-ClpP protease (class III stress gene)
locus
BSU13700
pI
5.14
mw
77.72 kDa
protein length
699 aa Sequence Blast
gene length
2097 bp Sequence Blast
function
protein degradation
product
AAA unfoldase, ATPase subunit of the ClpE-ClpP protease
essential
no
synonyms
ykvH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

    • Gene

    Coordinates
    1,435,628 → 1,437,727

    The protein

    Catalyzed reaction/ biological activity

  • ATPase/chaperone

    • Protein family

  • AAA -type ATPase (IPR013093) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR013093 InterPro] (PF07724) [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

    • Paralogous protein(s)

  • [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC], [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|ClpX]

    • [SW|Domains]

  • AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

    • [SW|Localization]

  • localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] [http://www.ncbi.nlm.nih.gov/pubmed/18786145 Pubmed]
  • forms foci coincident with nucleoid edges, usually near cell poles [Pubmed|18689473]
  • [[File:ClpE.jpg‎ ]]

    • Expression and Regulation

    Operons

    genes
    [gene|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE]
    description
    [Pubmed|10320580]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10320580], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|10320580,9987115,11179229,16163393,17380125], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]

    • regulation

  • induced by heat ([protein|search|CtsR]) [Pubmed|10320580,9987115]
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    Biological materials

    Mutant

  • ''clpE::spec'' available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab
  • BKE13700 (Δ[gene|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCCAAAACCTCCTTA, downstream forward: _UP4_TAACAATCAGCGGTTTCCTT
  • BKK13700 (Δ[gene|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCCAAAACCTCCTTA, downstream forward: _UP4_TAACAATCAGCGGTTTCCTT

    • GFP fusion

  • C-terminal YFP and CFP fusions (single copy) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1799 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs

    • Labs working on this gene/protein

  • [SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]

    • References

    Reviews

  • 26639779,28748186

    • Original Publications

  • 17302811